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KMID : 1145120200180030283
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Asian Journal of Beauty and Cosmetology
2020 Volume.18 No. 3 p.283 ~ p.295
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Antioxidant Activity, Whitening and Anti-wrinkle Effects of Leaf and Seed Extracts of Brassica juncea L. Czern.
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Lee Jeong-Eun
Kim Ae-Jung
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Abstract
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Purpose : This study is to evaluate biological activity of leaf and seed extracts of Brassica juncea L and examine their utility value as cosmetic ingredients.
Methods : Total polyphenol, total flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity, and inhibition activities of tyrosinase and elastase were measured to evaluate biological activity of leaf and seed extracts of Brassica juncea L. Czern.(EtOH). In addition, to investigate the antioxidant effect and enzyme inhibition activities in relation to concentrations, correlation of different concentrations of 50, 100, and 200 mg/mL of each extract were comparatively analyzed.
Results : The highest total polyphenol contents of leaf and seed extracts of Brassica juncea L (50, 100, and 200 mg/mL) were 15.03 and 27.72 mg TAE/g, respectively, while the total flavonoid contents were 58.84 and 126.75 mg QE/g, respectively. The DPPH scavenging effect was confirmed as 65.26% in leaf extracts and 83.17% in seed extracts, while the ABTS radical scavenging effect was confirmed as 47.06% in leaf extracts and 60.57% in seed extracts. As a result of evaluation of biological activity analysis on antioxidant effect, the activity in seed was higher than the activity in leaf, and a positive correlation was indicated as highest activities were shown in the concentration of 200 mg/mL for both leaf and seed. The highest inhibition activities of tyrosinase were 22.33% and 36.58%, respectively, while the inhibition activities of elastase were 33.45% and 40.21%, respectively. In leaf extracts, the inhibition activities of tyrosinase and elastase increased as the concentration increased, while in seed extract, the inhibition activities of elastase increased as the concentration increased, but the inhibition activities decreased when the concentration of the extract reached above a certain level.
Conclusion : The results of the study confirmed leaf and seed extracts of Brassica juncea L. Czern. have excellent antioxidant effect and inhibition effect on activities of tyrosinase and elastase, and their utility value as cosmetic ingredients would be high.
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KEYWORD
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Brassica juncea L, Antioxidant activity, Tyrosinase inhibitory activity, Elastase inhibitory activity, Bioactivity
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